Title : SID - 1 functions in multiple roles to support parental RNAi in Caenorhabditis
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چکیده
Title page Classification: Biological sciences, Genetics Title: SID-1 functions in multiple roles to support parental RNAi in Caenorhabditis elegans Authors: Eddie Wang, Craig P. Hunter Author affiliation: Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138 Corresponding author: Craig P. Hunter, 16 Divinity Avenue Room 3044, Cambridge, MA 02138. (617)495-8305. [email protected]
منابع مشابه
SID-1 Domains Important for dsRNA Import in Caenorhabditis elegans
In the nematode Caenorhabditis elegans, RNA interference (RNAi) triggered by double-stranded RNA (dsRNA) spreads systemically to cause gene silencing throughout the organism and its progeny. We confirm that Caenorhabditis nematode SID-1 orthologs have dsRNA transport activity and demonstrate that the SID-1 paralog CHUP-1 does not transport dsRNA. Sequence comparison of these similar proteins, i...
متن کاملSID-1 domains important for dsRNA import in C. elegans
In the nematode Caenorhabditis elegans, RNA interference (RNAi) triggered by doublestranded RNA (dsRNA) spreads systemically to cause gene silencing throughout the organism and its progeny. We confirm that Caenorhabditis nematode SID-1 orthologs have dsRNA transport activity and demonstrate that the SID-1 paralog CHUP-1 does not transport dsRNA. Sequence comparison of these similar proteins, in...
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Systemic RNAi in Caenorhabditis elegans requires the widely conserved transmembrane protein SID-1 to transport RNAi silencing signals between cells. When expressed in Drosophila S2 cells, C. elegans SID-1 enables passive dsRNA uptake from the culture medium, suggesting that SID-1 functions as a channel for the transport of double-stranded RNA (dsRNA). Here we show that nucleic acid transport by...
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Double-stranded RNA-mediated gene interference (RNAi) in Caenorhabditis elegans systemically inhibits gene expression throughout the organism. To investigate how gene-specific silencing information is transmitted between cells, we constructed a strain that permits visualization of systemic RNAi. We used this strain to identify systemic RNA interference-deficient (sid) loci required to spread ge...
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Conditional knockout (cKO) based on site-specific recombination (SSR) technology is a powerful approach for estimating gene functions in a spatially and temporally specific manner in many model animals. In Caenorhabditis elegans (C. elegans), spatial- and temporal-specific gene functions have been largely determined by mosaic analyses, rescue experiments and feeding RNAi methods. To develop a s...
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تاریخ انتشار 2017